12/16/2023 0 Comments Bd flow cytometry![]() ![]() ![]() 553057) and Hamster Anti-Mouse CD28 (Cat. 562158), washed twice, and then cultured (3 days) with Purified NA/LE Hamster Anti-Mouse CD3e (Cat. BALB/c splenocytes were stained (10 min, 37☌) with BD Horizon™ Violet Proliferation Dye 450 (Cat. Analysis of proliferating mouse splenocytes for surface and intracellular markers. Flow cytometry was performed using a BD™ LSRII Cell Analyzer System. Histograms were derived from gated events with the light scattering characteristics of Jurkat cells. 554655) and permeabilized in Perm/Wash Buffer I (Cat. Cells were either not fixed (solid line histograms), or fixed in BD Cytofix™ Fixation Buffer (Cat. Human Jurkat cells were treated (16 hr) with 0.025% DMSO (Top Plot) or 5 μM camptothecin (Bottom Plot) and stained with BD Horizon™ Fixable Viability Stain 780 (Cat. Fluorescent staining of Jurkat cells with BD Horizon™ Fixable Viability Stain 780. The service manager, and staff authorized by him, will assist users in experimental planning/design, development of acquisition protocols, sample acquisition, and data processing.Ī special web link will be prepared for booking.Figure 1. More details can be found at the following linkĭirect access to the instrumentation (flow cytometers) is restricted to personnel of the Department of Life Sciences with proven training and experience in the field of flow cytometry. violet (405 nm), blue (488nm) and red (640) allowing detection of up to 9 colors. Helps achieve scientific insights, laboratory efficiency and cost savings Offers advanced automation technology with BD FACSChorus ™ software and guides the user through the entire cell sorting process with its intuitive interface, on-screen instructions and prompts, and easy-to-read reportsĬombines sorter's proven and unique BD technology with automation and simplified software See the following links for more details.īD FACSMelody makes the complex world of flow cytometry and sorting accessible to more researchers. The system is equipped with a 488nm blue laser allowing detection of up to 6 parameters. It makes multi-parameter flow cytometry available to all researchers, ensuring consistent and reproducible data even on difficult samples or underrepresented populations. The Department of Life Sciences has the following instrumentation:Īttune NxT® Flow Cytometer from Thermofisher® is a compact analysis system with innovative hydrodynamic focusing technology. studies of the mechanism of action of membranolytic substances toward gram+ and gram- bacteria.analysis of cellular functions (phagocytosis, endocytosis, ROS).analysis of markers (membrane or cytosolic).It enables applications in biomedical and environmental fields, such as: It is a rapid and sensitive technique that can provide information on both eukaryotic and prokaryotic cells. It provides for a fee, to be paid by the head of the requesting research group, to support the operating/maintenance costs of the instruments, as indicated in the fee schedule described in the regulations and summarized in the cost table (see bottom of page).įlow cytometry is a technique that allows qualitative and quantitative analysis of single cells. Flow cytometry services, established in the Department of Life Sciences, make expertise and tools available to users inside and outside the University.Īccess to the services is subject to regulation (see bottom of page). ![]()
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